References Raman spectroscopy has an intrinsic sensitivity to the chemical content of the bone, but its application to study bones in vivo is limited due to strong optical scattering in tissue. low frequency Raman scattering ofCdS 1-xSe x, only the spheroidal modes ofl=0 (symmetrical mode) and forl=2 (quadrupole mode) are observed. It is important, since, unlike spontaneous Raman scattering spectroscopy, which provides with the whole vibrational spectrum for a single wavelength excitation, stimulated Raman scattering excitation is limited to a single vibrational line. A very common pulsed optical source is a frequency-doubled Q-switched Nd:YAG laser. These huge increases in Raman scattering are primarily caused by the increased intensity of the electromagnetic … The Journal of Biomedical Optics (JBO) is an open access journal that publishes peer-reviewed papers on the use of novel optical systems and techniques for improved health care and biomedical research. The development of a stimulated Raman scattering photoacoustic (SRS-PA) spectrometer is presented. Multimodality Raman and photoacoustic imaging of surface-enhanced-Raman-scattering-targeted tumor cells Wei Shi, aRobert J. Paproski, a,b Peng Shao, Alexander Forbrich, a John D. Lewis, b and Roger J. Zemp a,* a University of Alberta, Department of Electrical and Computer Engineering, Second Floor ECERF, 9107-116 Street, We demonstrate the feasibility of developing such an optical imaging modality by combining the molecularly specific stimulated Raman excitation with the photoacoustic detection. Photoacoustic imaging is a novel imaging technology for visualizing optically-absorbing structures in vivo with simultaneous high contrast and high spatial resolution. United States Patent 4197009 . photoacoustic Raman spectroscopy Yuanqin Yu, ... ulated Raman scattering process occurs, and the molecules are populatedfrom the ground vibrational level to an excited vibrational level. The molecular population at this excited level will be reduced by the subsequent collision-induced vibrational relaxation. The Raman scattering amplification associated with this phenomenon is known as surface-enhanced Raman scattering (SERS) spectroscopy . In situ Raman scattering of graphene further confirms that the temperature of graphene under laser pulses rises above the boiling point of water but still remains too low to vaporize graphene and create graphene plasma bubbles. Normal pure vibrational Raman scattering. Photoacoustic imaging employing molecular overtone vibration as a contrast mechanism opens a new avenue for bond-selective imaging of deep tissues. A tuning mechanism 14 adjusts the frequency difference between the radiation beams 15, 17 to equal substantially the vibrational … The sensitivity of a new spectroscopic technique, photoacoustic Raman spectroscopy (PARS), has been improved substantially by the use of high-peak-power pulsed lasers. Abstract: Photoacoustic microscopy (PAM) provides excellent image contrast based on optical absorption. Multimodality Raman and photoacoustic imaging of surface-enhanced-Raman-scattering-targeted tumor cells Wei Shi, aRobert J. Paproski,a,b Peng Shao, Alexander Forbrich,a John D. Lewis,b and Roger J. Zempa,* aUniversity of Alberta, Department of Electrical … Photoacoustic spectroscopy is the measurement of the effect of absorbed electromagnetic energy (particularly of light) on matter by means of acoustic detection. Conjugation of Raman reporter 4‐aminothiophenol (4‐ATP) onto AuNFs yields a surface‐enhanced Raman scattering (SERS) fingerprint for Raman spectroscopy/imaging. Photoacoustic Raman spectroscopy . 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